MM537

mM537 is a human melanoma derived from a metastatic site (lymph node). Cell Bank Australia reference number CBA-1356.

Human melanoma; from metastatic site - lymph node

Not specified

RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + 10% FBS.

Optimal split ratio 1:4 (minimum seeding density 2.7 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 1-2 min. MM537 required growth medium to be changed 3 times each week. The cells are sensitive to trypsin so exposure time to trypsin should be kept to a minimum. The cells take 2-3 days to recover from trypsinisation.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.