biological source
Mouse x Mouse Hybridoma
manufacturer/tradename
ECACC
packaging
tube of 5 μg 13082201-DNA-5UG, pkg of vial of cells 13082201-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
P3-NS1/1-Ag4.1
Reactivity
Human ′A′ erythrocytes
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Immunogen
Human tonsil membrane
Application
Human erythrocyte marker.
Biochem/physiol Actions
The W6/1 hybridoma cell line secretes monoclonal IgM antibodies reactive with a polymorphic determinant of human erythrocytes which correlated with blood group A.
Preparation Note
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)
Seed at 5 x 104 cells/ml. Subculture when cells reach 5 x 105 -1 x 106 cells/ml (usually after 3-4 days), 5% CO2, 37°C. Start in 20% FBS when recovering from frozen.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.