biological source
Rat x Rat Hybridoma
packaging
tube of 5 μg 13082227-DNA-5UG, pkg of vial of cells 13082227-1VL
ImmunoDon
Rat DA spleen
Myeloma
Y3
Reactivity
Mouse CD147
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Immunogen
Recombinant CD147
Application
The isotype of the monoclonal antibody produced by this cell line is rat IgG1.
Biochem/physiol Actions
The hybridoma cell line OX-114 recognises mouse CD147 secretes monoclonal IgG1 antibodies reactive with rat L-selectin.
Preparation Note
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)
Seed at 5 x 104 cells/ml (5 x 105 cells/ml at resuscitation). Subculture when cells reach 5 x 105 - 1 x 106 cells/ml (usually after 3-4 days), 5% CO2, 37°C. 20% FBS can be used at resuscitation.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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