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主要文件

COO原产地证书/COA分析证书

MRC-5 SV2

Name: MRC-5 SV2
Brand: SIGMA

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 84100401, human lung (fetal), Fibroblast

别名:

MRC-5V2 Cells, MRC5V2 Cells

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About This Item

UNSPSC代码:

41106514

生物来源

human lung (fetal)

包装

tube of 5 μg 84100401-DNA-5UG
pkg of vial of cells 84100401-1VL

生长模式

Adherent

核型

Modal no. = 60-80, hyperdiploid

形态学

Fibroblast

产品

Not specified

受体

Not specified

技术

cell culture | mammalian: suitable

运输

dry ice

储存温度

−196°C

细胞系来源

Human foetal lung, SV40 transformed

细胞系描述

Derived from the parental cell line MRC-5 by transformation with the SV40 virus. The cells do not senesce.

应用

Transformation and virus studies: SV40

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 8
TPOX: 8
vWA: 15

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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