MDCK Cell Line canine
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 84121903, canine kidney, Epithelial
别名:
MDCK (NBL-2) Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
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About This Item
UNSPSC代码:
41106514
eCl@ss:
32190102
生物来源
canine kidney
表单
cell suspension
包装
tube of 5 μg 84121903-DNA-5UG
pkg of vial of cells 84121903-1VL
生长模式
Adherent
核型
2n = 78
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
细胞系来源
Canine Cocker Spaniel kidney
细胞系描述
From kidney of normal female adult Cocker Spaniel in 1958 by SH Madin and NB Darby (Madin Darby Canine Kidney). Supports growth of wide range of animal viruses: VSV (Indiana strain), infectious Canine Hepatitis, Vaccinia, Coxsackie B5, Adeno and reo viruses, SVEV.
应用
Virus studies: SVEV, VSV, Vaccinia, adeno and reoviruses, Coxsackie B5 et al
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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