biological source
mouse embryo
packaging
tube of 5 μg 85011403-DNA-5UG, pkg of vial of cells 85011403-1VL
growth mode
Suspension
karyotype
n = 40
morphology
Teratocarcinoma
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Differentiation and tumourigenicity studies
Biochem/physiol Actions
Mouse SIM embryo teratocarcinoma
Thioguanine and ouabain resistant derivative of SIM. When cultured in absence of STO feeder cells it is stimulated to develop cell aggregates which differentiate resulting in formation of layer of endodermal cells covering aggregates.
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Requires STO feeder layer.
Other Notes
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法规信息
低风险生物材料
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