biological source
mouse breast
packaging
tube of 5 μg 85011406-DNA-5UG, pkg of vial of cells 85011406-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Cytotoxicity and tumourigenicity studies
Biochem/physiol Actions
From DBA/2 mouse tumour treated with methylcholanthrene. Used as target cell for cytotoxic T-cell assays. Cells exhibit no effector activity in antibody dependent cell mediated cytotoxic system. Growth not inhibited by dextran sulphate, LPS or PPD.
Mouse DBA/2 mastocytoma
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml. The cells are non-adherent but can give rise to adherent cells with large granules; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.