L929 Cell Line from mouse

Virus studies: PRV, VSV (Indiana), HSV

Mouse C3H/An connective tissue

Subclone of parental strain L, established by W R Earle in 1940. One of the first to be established in continuous culture. The L strain was derived from normal subcutaneous areolar and adipose tissue of a 100 day old male C3H/An mouse. Cells are APRT+ and HPRT+.

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split semi-confluent cultures seeding at 5x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.