J774.2 Cell Line from mouse

J774.2 Cell Line from mouse has been used:

• to study the effect of the growth hormone secretagogue peptide A233 on J774.2 cell line
• to study the ability of Mycobacterium tuberculosis clinical isolates to penetrate and grow inside the J774.2 cell line
• to study the effect of ivy leaf dry extract EA 575 on the lipopolysaccharide-induced release of interleukin-6 (IL-6) from J774.2 cell line

Mouse BALB/c monocyte macrophage

Recloned from J774.1 original ascites and solid tumour. Produces IL-1. Cell line J774.2 was recloned from the original ascites and solid tumor J774.1, and produces interleukin-1 (IL-1).

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cells are semi-adherent i.e. some cells grow in suspension , some loosely attach to the surface and others flattened out and attach to the flask. They should be passaged by scraping. Cells should not be allowed to overgrow and become confluent as this can lead to a loss of the flattened adherent cell characteristic.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.