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Merck
CN

MDCK

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85011435, dog kidney, Epithelial-like

别名:

MDCK1 Cells, MDCKI Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells

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关于此项目

eCl@ss:
32190102
UNSPSC Code:
41106514
Biological source:
dog kidney
Growth mode:
Adherent
Karyotype:
2n = 78
Morphology:
Epithelial-like

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biological source

dog kidney

packaging

tube of 5 μg 85011435-DNA-5UG, pkg of vial of cells 85011435-1VL

growth mode

Adherent

karyotype

2n = 78

morphology

Epithelial-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

相关类别

Application

Virus studies: SVEV, VSV, Vaccinia, reoviruses and inf. canine hepatitis virus

Biochem/physiol Actions

Canine Cocker Spaniel kidney
Derived from the kidney of a normal adult Cocker Spaniel in 1958. Useful in viral studies, supports the growth of vesicular exanthema virus of swine, vesicular stomatitis, vaccina, coxsackie B-5, adenoviruses, reoviruses and infectious canine hepatitis. Also known as MDCK (NBL-2).

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

低风险生物材料
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历史批次信息供参考:

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