生物来源
human lung (fetal)
包装
tube of 5 μg 85020203-DNA-5UG
pkg of vial of cells 85020203-1VL
生长模式
Adherent
核型
2n = 46, diploid
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
细胞系来源
Human foetal lung
细胞系描述
Established from foetal lung tissue. The cells have a finite lifespan and a similar virus susceptibility to the line MRC-5.
应用
Virus studies: rhinoviruses
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他说明
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