biological source
mouse blood
packaging
tube of 5 μg 85022106-DNA-5UG, pkg of vial of cells 85022106-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Not specified
products
Immunoglobulin A lambda
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Established in vitro from the MOPC 315 plasmacytoma induced by mineral oil injections in a BALB/c mouse. Secretes an IgA lambda antibody which recognises DNP-TNP substituted proteins. Cells are killed by low concentrations (10nM) of dexamethasone.
Mouse myeloma
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10-20% Foetal Bovine Serum (FBS).
Maintain cultures between 1-9x100,000 cells/ml; 5% CO2; 37°C. Do not allow cell density to rise above 1,000,000 cells/ml as rapid death occurs at higher concentrations.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.