3T3 Swiss Albino
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85022108, mouse embryo, Fibroblast
生物来源
mouse embryo
包装
tube of 5 μg 85022108-DNA-5UG
pkg of vial of cells 85022108-1VL
生长模式
Adherent
核型
2n = 40; hypertriploid
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
一般描述
Product has lost contact inhibition
应用
Contact inhibition and virus studies: polyoma and SV40 viruses
生化/生理作用
Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition. Cells have now lost their contact inhibition.
Mouse Swiss Albino embryo fibroblast
制备说明
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
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