biological source
mouse nerve
packaging
tube of 5 μg 85042301-DNA-5UG, pkg of vial of cells 85042301-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Glial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
Ethyl nitrosourea induced tumour in C3H. The mice, originated in trigeminal nerve and brain; readily transplanted and resembles schwannoma histologically. Cultured cells form prominent processes, resemble 33B (Sigma Catalogue number. 85081901) morphologically, contain abundant C type particles.
Mouse C3H.He Schwannoma
Preparation Note
DMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.