H9

Detection, isolation and propagation of HIV-1

Clonal derivative of the T-cell line HUT 78 derived from the blood of a patient with Sezary syndrome. The clone was selected due to its suitability for the isolation and continuous high level production and HIV variants with patients within AIDS and pre-AIDS. Examination with DNA probes indicate identical HLA typing and DNA fingerprints as HUT-78 .

Human T cell

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,12
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9
TPOX: 8,9
vWA: 14,15

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.