biological source
mouse blood
packaging
tube of 5 μg 85051501-DNA-5UG, pkg of vial of cells 85051501-1VL
growth mode
Semi-adherent
karyotype
Not specified
morphology
Macrophage
products
Not specified
receptors
Immunoglobulin, complement
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Adapted to culture from a spontaneous BALB/c mouse lymphoid tumour. Thought to be of B lymphocyte origin. Resistant to dextran sulphate. Produces isoenzyme and is capable of antibody-dependent lysis of sheep erythrocytes and tumour targets.
Mouse BALB/c lymphoid macrophage
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Detach cells by aspirating or gentle shaking.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.