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Merck
CN

HT29/219

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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关于此项目

UNSPSC Code:
41106514
Biological source:
human colon
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 46, hypertriploid
Morphology:
Epithelial

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产品名称

HT29/219, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

biological source

human colon

packaging

tube of 5 μg 85061109-DNA-5UG
pkg of vial of cells 85061109-1VL

growth mode

Adherent

karyotype

2n = 46, hypertriploid

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

相关类别

Biochem/physiol Actions

Human Caucasian colon carcinoma
Subclone of parent HT-29 which was established from an adenocarcinoma from a 44 year old Caucasian female. Tumorigenic in nude mice and has ability to grow in agar.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

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