biological source
human lymph
packaging
tube of 5 μg 85062003-DNA-5UG, pkg of vial of cells 85062003-1VL
growth mode
Suspension
karyotype
Modal number 45 hypodiploid
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity and virus studies: EBV
Biochem/physiol Actions
Derived by in vitro infection of Ramos cells (Sigma Catalogue number. 85030802) with EBV. The line is EBV positive. The cells have B lymphocyte characteristics, with surface associated mu and kappa chains.
Human Caucasian Burkitt′s lymphoma
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 13,14
D16S539: 10,13
D5S818: 7,12
D7S820: 11
THO1: 7,9.3
TPOX: 8,9
vWA: 15,16
CSF1PO: 10,11
D13S317: 13,14
D16S539: 10,13
D5S818: 7,12
D7S820: 11
THO1: 7,9.3
TPOX: 8,9
vWA: 15,16
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.