生物来源
human lung (fetal)
包装
tube of 5 μg 85062512-DNA-5UG
pkg of vial of cells 85062512-1VL
生长模式
Adherent
核型
2n = 46, hyperdiploid, modal no. 73-78
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Human Caucasian foetal lung, SV40 transformed
细胞系描述
An SV40 transformed derivative of WI 38. The cells are characterised by loss of contact inhibition, unlimited proliferation and the presence of SV40 antigens.
应用
Transformation and virus studies: SV40
制备说明
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他说明
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