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Merck
CN

WI 38VA13 Subline 2RA

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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关于此项目

UNSPSC Code:
41106514
Biological source:
human lung (fetal)
Growth mode:
Adherent
Karyotype:
2n = 46, hyperdiploid, modal no. 73-78
Morphology:
Epithelial

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biological source

human lung (fetal)

packaging

tube of 5 μg 85062512-DNA-5UG, pkg of vial of cells 85062512-1VL

growth mode

Adherent

karyotype

2n = 46, hyperdiploid, modal no. 73-78

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

相关类别

Application

Transformation and virus studies: SV40

Biochem/physiol Actions

An SV40 transformed derivative of WI 38. The cells are characterised by loss of contact inhibition, unlimited proliferation and the presence of SV40 antigens.
Human Caucasian foetal lung, SV40 transformed

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

常规特殊物品
低风险生物材料
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历史批次信息供参考:

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