RAW 264 Cell Line from mouse

RAW 264 Cell Line from mouse has been used:

• to study the anti-inflammatory effect of galectin-3 on RAW 264 macrophages which are stimulated with lipopolysaccharide
• to study the effect of titanium particles on progranulin (PGRN) expression in RAW 264 cells
• to study the effects of a nutritional supplement on interferon-γ (IFN-γ)-induced expression of macrophage chemoattractant protein-1 (MCP-1) and intercellular adhesion molecule-1 (ICAM-1)

Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia virus (A-MuLV). Cells with pinocytose neutral red and phagocytose zymosan. Cells capable of antibody-dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS (0.5ng/ml).

Mouse leukaemic monocyte-macrophage

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm²; 5% CO₂; 37°C. Remove the cells mechanically. Cells are semi-adherent, i.e. some cells grows in suspension, some loosely attach to the surface and others flattened out and attach to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.

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