OAW42 has been used to investigate the cytotoxic potential of histone deacetylase 1 as a target for ovarian cancer immunotherapy and to study the effects of tazemetostat, a potent and selective EZH2 inhibitor, on OAW42 cell lines.

From an ascites of patient with ovarian cystadenocarcinoma. Has retained ability to form free floating cysts in vitro and produces extracellular matrix. Has defined chemosensitivity pattern. Valuable for studies on biology of human ovarian cancer.

Human ovarian tumour epithelial

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 12,13
D5S818: 11,12
D7S820: 8
THO1: 6,7
TPOX: 8,11
vWA: 15,16

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 20 IU/l Bovine Insulin + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

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