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Merck
CN

BW5147.G.1.4.OUA/R.1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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关于此项目

UNSPSC Code:
41106514
Biological source:
mouse blood
Relevant disease(s):
cancer
Growth mode:
Suspension
Morphology:
Lymphoblast

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biological source

mouse blood

packaging

tube of 5 μg 85082302-DNA-5UG, pkg of vial of cells 85082302-1VL

growth mode

Suspension

morphology

Lymphoblast

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

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General description

For complete product information, please see BW5147.G.1.4.OUA/R.1

Application

Fusion partner

Biochem/physiol Actions

Mouse AKR/J T cell lymphoma
This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100μM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.

Preparation Note

Maintain cultures between 1-6x100,000 cells/ml; 5% CO2; 37°C.
SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2mM Sodium Pyruvate (NaP) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

低风险生物材料
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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