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A431 Cell Line human

Name: A431 Cell Line human
Brand: SIGMA

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85090402, human skin, Epithelial

别名:

A-431 Cells, A431/P Cells

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About This Item

UNSPSC代码:

41106514

生物来源

human skin

包装

tube of 5 μg 85090402-DNA-5UG
pkg of vial of cells 85090402-1VL

生长模式

Adherent

核型

Hypertriploid

形态学

Epithelial

产品

Not specified

受体

Epidermal growth factor (EGF)

技术

cell culture | mammalian: suitable

运输

dry ice

储存温度

−196°C

细胞系来源

Human squamous carcinoma

细胞系描述

Derived from an epidermal carcinoma of the vulva taken from an 85 yr old female. The cells carry large numbers of EGF binding sites and is an indicator cell for anti-TGF binding.

应用

A431 Cell Line human has been used:

to demonstrate that p38 mitogen-activated protein kinase enhances epidermal growth factor-induced apoptosis in A431 cells
to study melanoma-specific CD44 (a tumor marker and transmembrane receptor) alternative splicing pattern in A431 cells
to study the anti-cancer activity of antioxidant-rich Cameroonian medicinal plants

Cell cycle studies

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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