biological source
mouse blood
packaging
tube of 5 μg 85092601-DNA-5UG, pkg of vial of cells 85092601-1VL
growth mode
Suspension
karyotype
Aneuploid
morphology
Lymphoblast
products
Rauscher virus
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity and virus studies: Rauscher virus
Biochem/physiol Actions
A Rauscher virus induced erythroid leukaemia cell line. It is known to produce low amounts of Rauscher virus (a murine leukaemia virus).
Mouse leukaemic lymphoblast
Preparation Note
DMEM or RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Let the cell culture media become acidic before sub-culturing/freezing. Limited lifespan in vitro, therefore a master bank should be frozen as soon as possible, of at least 10 ampoules.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.