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T47D Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85102201, human breast, Epithelial

别名:

T-47-D Cells, T-47D Cells, T47D:A Cells

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关于此项目

UNSPSC Code:
41106514
Biological source:
human breast
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 46, hypertriploid, modal no. 65
Morphology:
Epithelial

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biological source

human breast

description

Human breast tumor

packaging

tube of 5 μg 85102201-DNA-5UG, pkg of vial of cells 85102201-1VL

growth mode

Adherent

karyotype

2n = 46, hypertriploid, modal no. 65

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

相关类别

Application

T47D cell line human has been used:
  • to study the effects of a polysaccharide–protein complex (PPC) isolated from metabolites of gut bacteria Raoultella ornithinolytica from the earthworm Dendrobaena veneta against Candida albicans
  • has been cultured and incubated with bone marrow-derived macrophages to study their migration and podosome formation
  • to study the anti-proliferative effects of two novel classes of potential antimetabolites on T47D cells

Biochem/physiol Actions

Established from the pleural effusion of a ductal carcinoma of the breast of a 54-year-old female. The cells carry receptors for a variety of steroids.
Human breast tumour
STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 12
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 6
TPOX: 11
vWA: 14

Preparation Note

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

低风险生物材料
常规特殊物品
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分析证书(COA)

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