生物来源
human breast
描述
Human breast tumor
包装
tube of 5 μg 85102201-DNA-5UG
pkg of vial of cells 85102201-1VL
生长模式
Adherent
核型
2n = 46, hypertriploid, modal no. 65
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Human breast tumour
细胞系描述
Established from the pleural effusion of a ductal carcinoma of the breast of a 54-year-old female. The cells carry receptors for a variety of steroids.
应用
T47D cell line human has been used:
- to study the effects of a polysaccharide–protein complex (PPC) isolated from metabolites of gut bacteria Raoultella ornithinolytica from the earthworm Dendrobaena veneta against Candida albicans
- has been cultured and incubated with bone marrow-derived macrophages to study their migration and podosome formation
- to study the anti-proliferative effects of two novel classes of potential antimetabolites on T47D cells
DNA图谱分析
STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 12
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 6
TPOX: 11
vWA: 14
CSF1PO: 11,13
D13S317: 12
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 6
TPOX: 11
vWA: 14
培养基
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他说明
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