biological source
mouse adipose tissue
packaging
tube of 5 μg 85103115-DNA-5UG, pkg of vial of cells 85103115-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Derived from normal subcutaneous connective tissue of an adult C3H strain mouse. The cells were treated with 20 methylcholanthrene and produced sarcomas when injected into C3H strain mice.
Mouse C3H/An areolar and adipose tissue
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split semi-confluent cultures 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells /cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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法规信息
低风险生物材料
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