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Merck
CN

BRL 3A

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85111503, rat liver, Epithelial

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关于此项目

UNSPSC Code:
41106514
Biological source:
rat liver
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial

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biological source

rat liver

packaging

tube of 5 μg 85111503-DNA-5UG, pkg of vial of cells 85111503-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

MSA factors

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

相关类别

Application

Growth factor studies

Biochem/physiol Actions

Buffalo Rat liver
Originally isolated by primary cloning. Serum-free medium conditioned by the cells produces a family of polypeptides termed multiplication stimulating activity (MSA).

Preparation Note

Coon′s modified Ham′s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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法规信息

低风险生物材料
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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