biological source
human lymph
packaging
tube of 5 μg 86010701-DNA-5UG, pkg of vial of cells 86010701-1VL
growth mode
Suspension
karyotype
2n = 46, hyperdiploid
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Clonally-derived sub-line of JIYOYE (Sigma Catalogue number. 88071302) containing high levels of EBV. Has been successfully hybridised with the human lymphoid cell line K562 to form the hybrid PUTKO-1. ACDP class II pathogen and should be handled under laboratory containment level 2. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Human Burkitt′s Lymphoma
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 7,9
TPOX: 6,8
vWA: 15,19
CSF1PO: 10,11
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 7,9
TPOX: 6,8
vWA: 15,19
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Other Notes
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法规信息
常规特殊物品
低风险生物材料
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