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SK.N.SH Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86012802, human nerve (bone marrow metastasis; Caucasian neuroblastoma), Epithelial

别名:

NSH Cells, SK-NSH Cells, SKNSH Cells

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关于此项目

UNSPSC Code:
41106514
Biological source:
human nerve (bone marrow metastasis; Caucasian neuroblastoma)
Relevant disease(s):
metastasis
Growth mode:
Adherent
Karyotype:
2n = 46, modal no. 47
Morphology:
Epithelial

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biological source

human nerve (bone marrow metastasis; Caucasian neuroblastoma)

packaging

tube of 5 μg 86012802-DNA-5UG, pkg of vial of cells 86012802-1VL

growth mode

Adherent

karyotype

2n = 46, modal no. 47

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

metastasis

shipped in

dry ice

storage temp.

−196°C

相关类别

Application

Human SK.N.SH cell line has been used in dark toxicity testing of a chloro-indium-phthalocyanine photosensitizer (In-Pc). It has also been used to study the potential mechanisms and domain requirements of the hHSJ1a (DnaJ heat shock protein family member B2) mediated reduction in superoxide dismutase 1 (SOD1) aggregation.

Biochem/physiol Actions

Established from a bone marrow metastasis from a 4-year-old caucasian female suffering with neuroblastoma. The cells have been used as a target cell in cell-mediated cytoxicity tests.
Human Caucasian neuroblastoma
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

常规特殊物品
低风险生物材料
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商品

ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

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