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A549 Cell Line human

Name: A549 Cell Line human
Brand: SIGMA

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86012804, human lung (carcinoma), Epithelial

别名:

A-549 Cells, A549 Cells, NCI-A549 Cells

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关于此项目

UNSPSC Code:

41106514

Biological source:

human lung (carcinoma)

Relevant disease(s):

cancer

Growth mode:

Adherent

Karyotype:

Hypotriploid

Morphology:

Epithelial

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biological source

human lung (carcinoma)

packaging

tube of 5 μg 86012804-DNA-5UG, pkg of vial of cells 86012804-1VL

growth mode

Adherent

karyotype

Hypotriploid

morphology

Epithelial

products

Lecithin, High amounts of de-saturated fatty acids.

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

Human A549 cell line has been used:

to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions
to study the effects of insulin and insulin-like growth factor 1 (IGF1) on proliferation and apoptosis in A549 cells
to culture A549 cells in different media over a long term and identify conditions for promoting alveolar type II differentiation characteristics in A549 cells

Enzymatic studies

Biochem/physiol Actions

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

Human Caucasian lung carcinoma

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14

Preparation Note

Ham′s F12K + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or tTrypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

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