biological source
mouse blood
packaging
tube of 5 μg 86060302-DNA-5UG, pkg of vial of cells 86060302-1VL
growth mode
Semi-adherent
karyotype
Not specified
morphology
Lymphoblast
products
Interleukin 2 (IL-2)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
IL-1 assay
Biochem/physiol Actions
Derived from a lymphoma that occurred in a B10.BR mouse. The cells respond to Interleukin 1 (IL-1) stimulation by producing Interleukin 2 (IL-2) and can be used to assay IL-1 activity.
Mouse B10.BR lymphoma
Preparation Note
Maintain cultures between 5x100,000 to 1,000,000 cells/ml; 5% CO2; 37°C. Shake the flask to remove attached cells.
RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 15% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
法规信息
动物来源培养基
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