biological source
rat blood
packaging
tube of 5 μg 86061001-DNA-5UG, pkg of vial of cells 86061001-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Differentiation studies
Biochem/physiol Actions
Exhibits marked variations in differentiation when monitored for basophilic granule development, histamine content and membrane receptor sites for IgE.
Rat basophilic leukaemia granulocyte
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10-20% Foetal Bovine Serum (FBS).
Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 5% CO2; 37°C. Treat as suspension when sub-culturing. Some fibroblast-like cells within the population will attach. Please disregard these and subculture as per normal suspension cell lines.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.