biological source
rat blood
packaging
tube of 5 μg 86061001-DNA-5UG, pkg of vial of cells 86061001-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Differentiation studies
Biochem/physiol Actions
Exhibits marked variations in differentiation when monitored for basophilic granule development, histamine content and membrane receptor sites for IgE.
Rat basophilic leukaemia granulocyte
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10-20% Foetal Bovine Serum (FBS).
Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 5% CO2; 37°C. Treat as suspension when sub-culturing. Some fibroblast-like cells within the population will attach. Please disregard these and subculture as per normal suspension cell lines.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
低风险生物材料
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