CII

Somatic cell hybrid mapping

Human x mouse hybrid

This cell line may contain a sub-chromosomal fragment of human chromosome 17 containing markers thought at the time to flank the cystic fibrosis gene. The DNA was donated from the human osteosarcoma cell line MNNG-HOS. Used in chromosome mapping studies and somatic cell nutridisation.

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO₂.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.