biological source
human stomach (gastric carcinoma)
packaging
tube of 5 μg 86093004-DNA-5UG, pkg of vial of cells 86093004-1VL
growth mode
Suspension
karyotype
2n = 46, hypertriploid
morphology
Spherical
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Derived from a metastasis of a gastric carcinoma in a 55-year-old Down Syndrome male.
Human gastric carcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 7,11
D13S317: 8,12
D16S539: 10,12
D5S818: 10,11
D7S820: 8,12
THO1: 7,9
TPOX: 11
vWA: 14,16
CSF1PO: 7,11
D13S317: 8,12
D16S539: 10,12
D5S818: 10,11
D7S820: 8,12
THO1: 7,9
TPOX: 11
vWA: 14,16
Preparation Note
Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C. Cells grow in suspension, but will increasingly to adhere to the surface during longer culture. For passaging it is recommended to subculture both cell types using scrapers for dislodging adh
RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.