KG-1

KG-1 has been used to extract total RNA and use them as templates to determine the 5′ end of promyelocytic leukemia zinc finger (PLZF) transcripts.

Derived from the bone marrow aspirate of a 59 year old male Caucasian with erythroleukemia that became acute myelogenous leukaemia. The cells form colonies in soft-agar in the presence of colony stimulating factor (CSF). KG-1 cells resemble acute myelogenous leukaemia showing considerable pleomorphism with a predominance of myeloblasts and promyelocytes. A small percentage of the cells are mature granulocytes with the occasional presence of macrophages and eosinophils. They do not express surface Ig or EBV antigens and lack specific markers for lymphocytic cells.

Human Caucasian bone marrow myelogenous leukaemia

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19

IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

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