biological source
human bone marrow
packaging
tube of 5 μg 86111306-DNA-5UG, pkg of vial of cells 86111306-1VL
growth mode
Suspension
karyotype
2n = 46, diploid
morphology
Myeloblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
KG-1 has been used to extract total RNA and use them as templates to determine the 5′ end of promyelocytic leukemia zinc finger (PLZF) transcripts.
Biochem/physiol Actions
Derived from the bone marrow aspirate of a 59 year old male Caucasian with erythroleukemia that became acute myelogenous leukaemia. The cells form colonies in soft-agar in the presence of colony stimulating factor (CSF). KG-1 cells resemble acute myelogenous leukaemia showing considerable pleomorphism with a predominance of myeloblasts and promyelocytes. A small percentage of the cells are mature granulocytes with the occasional presence of macrophages and eosinophils. They do not express surface Ig or EBV antigens and lack specific markers for lymphocytic cells.
Human Caucasian bone marrow myelogenous leukaemia
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19
Preparation Note
IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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法规信息
常规特殊物品
低风险生物材料
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