biological source
human blood
manufacturer/tradename
ECACC
packaging
tube of 5 μg 87012702-DNA-5UG, pkg of vial of cells 87012702-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Not specified
products
Immunoglobulin G (Ig) lambda light chain
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
RPMI 8226 has been used:
- to grow human myeloma cell lines
- to evaluate the effect of 1-aryl-2-methylthioimidazolines (IIV) on the viability of RPMI 8226 cells
- to measure cytokine production by bone marrow stromal cells (BMSCs) after contact with the RPMI 8226 cell line
Biochem/physiol Actions
Derived from the peripheral blood of a 61 year old male with multiple myeloma. The cells produce and secrete Ig lambda light chain.
Human myeloma
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. After resuscitation cell debris will be present in culture. This will decrease with subsequent subculture.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.