biological source
human blood
packaging
tube of 5 μg 87012703-DNA-5UG, pkg of vial of cells 87012703-1VL
growth mode
Suspension
karyotype
2n = 46
morphology
Lymphoblast
products
Immunoglobulin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
Human Caucasian lymphoblast
Initiated from the leukocytes obtained from a 32 year old Caucasian male having no known malignancy. EBV particles are present. The cells produce immunoglobulins in vitro.
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 9,11
D5S818: 11,12
D7S820: 8,11
THO1: 7,9.3
TPOX: 8,11
vWA: 14,16
CSF1PO: 11,12
D13S317: 8,12
D16S539: 9,11
D5S818: 11,12
D7S820: 8,11
THO1: 7,9.3
TPOX: 8,11
vWA: 14,16
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.