生物来源
human cervix (small bowel metastasis)
包装
tube of 5 μg 87020501-DNA-5UG
pkg of vial of cells 87020501-1VL
生长模式
Adherent
核型
Modal no. 78
形态学
Epithelial
产品
Beta Human chorionic gonadotropin (beta-hCG)
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
metastasis
运输
dry ice
储存温度
−196°C
细胞系来源
Human Caucasian cervical epidermoid carcinoma
细胞系描述
Derived from an epidermoid carcinoma of the cervix metastatic to the small bowel mesentery of a 40 year old Caucasian. Ca Ski cells secrete the β subunit of human chorionic gonadotropin (β-h CG), express tumour-associated antigen and G6PD type B. Ca Ski cells have been reported to contain an integrated human papilloma virus 16 genome (HPV-16 about 600 copies per cell) and HPV-18 related sequences.
应用
Tumourigenicity studies
DNA图谱分析
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8,12
D16S539: 11
D5S818: 13
D7S820: 8,11
THO1: 7
TPOX: 8
vWA: 17
CSF1PO: 10
D13S317: 8,12
D16S539: 11
D5S818: 13
D7S820: 8,11
THO1: 7
TPOX: 8
vWA: 17
培养基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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