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UNSPSC Code:
41106514
Biological source:
human skin
Growth mode:
Adherent
Karyotype:
2n = 46, A modal chromosome number 47 with trisomy for group 21 in 75% of the cells
Morphology:
Fibroblast
biological source
human skin
packaging
tube of 5 μg 87032602-DNA-5UG, pkg of vial of cells 87032602-1VL
growth mode
Adherent
karyotype
2n = 46, A modal chromosome number 47 with trisomy for group 21 in 75% of the cells
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Virus studies
Biochem/physiol Actions
Derived from the foreskin of a 2 month old Caucasian with Down Syndrome.
Human Caucasian, skin, Down syndrome
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Finite lifespan, up to 30 passages expected.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
法规信息
低风险生物材料
常规特殊物品
此项目有
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