biological source
rat liver
packaging
tube of 5 μg 87050701-DNA-5UG, pkg of vial of cells 87050701-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Carcinogenesis and tumourigenicity studies
Biochem/physiol Actions
Derived from the liver of a Wistar rat. Useful in quantitative carcinogenesis studies. Inoculation into new born rats is more sensitive than nude mice for detection of tumorigenicity of rat liver lines.
Rat Wistar liver hepatoma
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cells 1:3 to 1:6 i.e. 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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法规信息
低风险生物材料
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