Human Caucasian colon adenocarcinoma

Established from the lymph node of a 51 year old Caucasian male. The cells synthesise small quantities of CEA and are highly tumorigenic in nude mice. The established cell line consists of small spherical and bipolar cells resembling microvilli.The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

SW 620 cell line has been used:

• as an in vitro model to study key cellular processes required for metastasis. It has also been used to study the effects of transforming growth factor-β (TGF-β) pathway and heat shock protein 90 (Hsp90) on the growth of cells
• to study the relationship between zinc intake and risk of colon cancer
• to test for intrahepatic tumor cell growth after intraportal injection

Tumourigenicity studies

STR-PCR Data: Amelogenin: X
CSF1PO: 13,14
D13S317: 12
D16S539: 9,13
D5S818: 13
D7S820: 8,9
THO1: 8
TPOX: 11
vWA: 16

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; No CO₂; 37°C.

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