跳转至内容
Merck
CN

SW 620 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87051203, human colon, Epithelial

别名:

SW620 Cells

登录查看公司和协议定价

关于此项目

UNSPSC代码:
41106514

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

生物来源

human colon

描述

Human Caucasian colon adenocarcinoma

包装

tube of 5 μg 87051203-DNA-5UG
pkg of vial of cells 87051203-1VL

生长模式

Adherent

核型

2n = 46, hyperdiploid, modal no. 50

形态学

Epithelial

产品

Carcinoembryonic antigen (CEA)

受体

Not specified

技术

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

应用

SW 620 cell line has been used:
  • as an in vitro model to study key cellular processes required for metastasis. It has also been used to study the effects of transforming growth factor-β (TGF-β) pathway and heat shock protein 90 (Hsp90) on the growth of cells
  • to study the relationship between zinc intake and risk of colon cancer
  • to test for intrahepatic tumor cell growth after intraportal injection
Tumourigenicity studies

生化/生理作用

Established from the lymph node of a 51 year old Caucasian male. The cells synthesise small quantities of CEA and are highly tumorigenic in nude mice. The established cell line consists of small spherical and bipolar cells resembling microvilli.The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Human Caucasian colon adenocarcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 13,14
D13S317: 12
D16S539: 9,13
D5S818: 13
D7S820: 8,9
THO1: 8
TPOX: 11
vWA: 16

制备说明

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; No CO2; 37°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 文件 section.

如需帮助，请联系 客户支持

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持