biological source
human colon
packaging
tube of 5 μg 87061205-DNA-5UG, pkg of vial of cells 87061205-1VL
growth mode
Suspension
karyotype
Hyperdiploid, modal no. 53
morphology
Round & Refractile
products
Serotonin, epinephrine, NPP, Adrenocorticotropic hormone (ACTH) and PTH
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Hormone studies
Biochem/physiol Actions
Human Caucasian colon adenocarcinoma
Isolated from a moderately undifferentiated adenocarcinoma from the sigmoid colon of a 55 year old Caucasian female. The cells have double minute (DM) chromosomes.
STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12
D7S820: 9,12
THO1: 8,9
TPOX: 8,9
vWA: 15,18
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12
D7S820: 9,12
THO1: 8,9
TPOX: 8,9
vWA: 15,18
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. may semi-adhere to culture flask.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.