生物来源
human colon
包装
tube of 5 μg 87071006-DNA-5UG
pkg of vial of cells 87071006-1VL
生长模式
Adherent
核型
2n = 46, hypotriploid
形态学
Epithelial
产品
Carcinoembryonic antigen (CEA)
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Human Caucasian colon adenocarcinoma
细胞系描述
Derived from a grade II adenocarcinoma of the colon extending into the muscularis from a 73 year old Caucasian male. The cells exhibit brush borders and produce high levels of CEA.
应用
Tumourigenicity studies
培养基
L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; CO2 is not needed. Medium change after 4-5 days. 37°C.
其他说明
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