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Merck
CN

SW 403

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87071008, human colon, Epithelial

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关于此项目

UNSPSC Code:
41106514
Biological source:
human colon
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 46, triploid
Morphology:
Epithelial

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biological source

human colon

packaging

tube of 5 μg 87071008-DNA-5UG, pkg of vial of cells 87071008-1VL

growth mode

Adherent

karyotype

2n = 46, triploid

morphology

Epithelial

products

Carcinoembryonic antigen (CEA)

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

相关类别

Application

Tumourigenicity studies

Biochem/physiol Actions

Established from a grade III adenocarcinoma of the bowel of a 51 year old Caucasian female. The cells produce CEA and are tumorigenic in nude mice. Electron microscopy reveals prominent golgi apparatus.
Human Caucasian colon adenocarcinoma

Preparation Note

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; No CO2; 37°C. Cells do not reach confluency but will grow in small islands.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

常规特殊物品
低风险生物材料
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历史批次信息供参考:

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