JW8/5/13

Mouse J558L + Mouse VH gene + Human C (epsilon) gene

This human chimeric antibody is directed against the hapten 4-hydroxy-3 nitrophenacetyl. The V lambda of the endogenous light chain of the plasmacytoma line complements the VNP of the transfected heavy chain to yield the specific antibody. Gene marker: gpt.

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.