生物来源
mouse skin
包装
tube of 5 μg 87081806-DNA-5UG
pkg of vial of cells 87081806-1VL
生长模式
Adherent
核型
Hypertetraploid
形态学
Epithelial
产品
Melanin
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Mouse (CxDBA)F1 melanoma
细胞系描述
Established from a Cloudman S91 melanoma in a male mouse; the cells produce melanin.
应用
Cloning studies
培养基
Ham′s F10 + 2mM Glutamine + 10% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS) or 15% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他说明
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