RPMI 6666

Tumourigenicity studies

Derived from a 29 year old male Caucasian with Hodgkin′s disease. One of a large series of permanent lines from leukocytes in the peripheral blood of donors with and without malignancies. Has cell-associated EBV and produces immunoglobulins. This cell line was initially thought to have been derived from Hodgkin lymphoma, but is now known to be an EBV-positive B-lymphoblastoid cell line.

Human Caucasian lymphoblast, Hodgkins disease

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.