生物来源
bovine trachea
包装
tube of 5 μg 87090202-DNA-5UG
pkg of vial of cells 87090202-1VL
生长模式
Adherent
核型
2n = 60
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Bovine foetal trachea
细胞系描述
Derived from the trachea of a male bovine foetus. The cell line can be used for isolation, replication and quantitation of BVDV, IBRV and parainfluenza type 3 virus. Growth and morphological characteristics decline beyond passage number 55.
应用
Virus studies: BVDV, IBRV and parainfluenza type 3 virus.
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他说明
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