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SW480 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87092801, human colon, Epithelial

别名:

SW-480 Cells, SW480E Cells

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关于此项目

UNSPSC Code:
41106514
Biological source:
human colon
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial

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biological source

human colon

description

Human colon adenocarcinoma

packaging

tube of 5 μg 87092801-DNA-5UG, pkg of vial of cells 87092801-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

相关类别

Application

Human SW480 cell line has been used to study the antiproliferative effects of F. mughlea Pesmen on cancer cells. It has also been used as an in vitro model to study key cellular processes required for metastasis. The cell line is used to study the effects of the transforming growth factor-β (TGF-β) pathway and heat shock protein 90 (Hsp90) on the growth of cells.

Biochem/physiol Actions

Derived from a grade 3-4 colon adenocarcinoma. The initial cultures contained a mixture of epithelial and bipolar cells, but subsequently epithelial cells predominated. The cells produce CEA.
Human colon adenocarcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 13,14
D13S317: 12
D16S539: 13
D5S818: 13
D7S820: 8
THO1: 8
TPOX: 11
vWA: 16

Preparation Note

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin; 37°C. Medium change every 4-5 days. CO2 is not required.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

低风险生物材料
常规特殊物品
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商品

ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

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