biological source
mouse blood
packaging
tube of 5 μg 87092804-DNA-5UG, pkg of vial of cells 87092804-1VL
growth mode
Suspension
karyotype
2n=40, diploid
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Established from a tumour induced in an 8 month old female 234 subline 212 of a DBA mouse. Orginally subcultured in DBA/2 mice it was adapted to suspension culture. The line has been extensively used in cytotoxicity assays. Develops massive tumours in nude mice after a latent period of 7-10 days
Mouse DBA/2 lymphocytic leukaemia
Preparation Note
Fischer′s medium + 2mM Glutamine + 10% Horse Serum (HS).
Maintain cultures between 5x10,000 to 8x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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